CHIDULE NDI KUTANTHAUZA MAYESERO
Matenda a typhoid amayamba ndi S. typhi, kachilombo ka Gram-negative.Padziko lonse lapansi, pafupifupi 17 miliyoni amafa ndi 600,000 omwe amafa nawo chaka chilichonse.Odwala omwe ali ndi kachilombo ka HIV amakhala pachiwopsezo chowonjezeka cha matenda a S. typhi.Umboni wa matenda a H. pylori umaperekanso chiopsezo chotenga typhoid fever.1-5% ya odwala amakhala chonyamulira aakulu okhala S. typhi mu ndulu.
Kuzindikira matenda a typhoid fever kumadalira kudzipatula kwa S. typhi ku magazi, m'mafupa kapena zilonda zinazake za anatomic.M'malo omwe sangakwanitse kuchita njirayi yovuta komanso yowononga nthawi, mayeso a Filix-Widal amagwiritsidwa ntchito kuti athandizire kuzindikira.Komabe, zofooka zambiri zimayambitsa zovuta pakutanthauzira mayeso a Widal.
Mosiyana ndi izi, Typhoid IgG/IgM Rapid Test Kit ndi mayeso osavuta komanso ofulumira a labotale.Kuyesako nthawi imodzi kumazindikira ndikusiyanitsa ma antibodies a IgG ndi IgM ku S. typhi yeniyeni antigen m'magazi athunthu motero amathandizira kudziwa zomwe zikuchitika kapena zam'mbuyomu za S. typhi.
MFUNDO
The Typhoid IgG/IgM Combo Rapid Test ndi lateral flow chromatographic
immunoassay.Kaseti yoyesera ili ndi: 1) burgundy colored conjugate pad yomwe ili ndi recombinant S. typhoid H antigen ndi O antigen conjugated ndi golidi wa colloid (typhoid conjugates) ndi kalulu IgG-golide conjugates, 2) nitrocellulose membrane strip yokhala ndi magulu awiri oyesera (M ndi G band).M band idakutidwa kale ndi monoclonal anti-human IgM kuti izindikire IgM anti-S.typhi, G band idakutidwa kale ndi ma reagents kuti azindikire IgG
anti-S.typhi, ndipo C band idakutidwa kale ndi anti rabbit IgG.
Pamene chiwerengero chokwanira cha chitsanzo choyesera chaperekedwa mu chitsime cha kaseti yoyesera, chitsanzocho chimasuntha ndi capillary action kudutsa kaseti.Anti-S.typhi IgM ngati ilipo mu chitsanzocho idzagwirizana ndi ma conjugates a typhoid.The immunocomplex kenako anagwidwa pa nembanemba ndi pre- TACHIMATA anti-anthu IgM antibody, kupanga burgundy mtundu M band, kusonyeza S. typhi IgM zotsatira zabwino mayeso.
Anti-S.typhi IgG ngati ilipo pachitsanzoyo idzalumikizana ndi ma conjugates a typhoid.The immunocomplex ndiye anagwidwa ndi pre- TACHIMATA reagents pa nembanemba, kupanga burgundy mtundu G gulu, kusonyeza S. typhi IgG zotsatira zabwino mayeso.
Kusakhalapo kwa magulu aliwonse oyeserera (M ndi G) kukuwonetsa zotsatira zoyipa.Mayesowa ali ndi chowongolera chamkati (C band) chomwe chimayenera kuwonetsa gulu lamtundu wa burgundy la immunocomplex of goat anti rabbit IgG/rabbit IgG-gold conjugate mosasamala kanthu za kukula kwa mtundu pamagulu aliwonse oyesa.Apo ayi, zotsatira zake ndizolakwika ndipo chitsanzocho chiyenera kuyesedwanso ndi chipangizo china.
