CHIDULE NDI KUTANTHAUZA MAYESERO
Malungo ndi matenda oyambitsidwa ndi udzudzu, hemolytic, febrile omwe amakhudza anthu opitilira 200 miliyoni ndipo amapha anthu opitilira 1 miliyoni pachaka.Zimayambitsidwa ndi mitundu inayi ya Plasmodium: P. falciparum, P. vivax, P. ovale, ndi P. malariae.Ma plasmodia onsewa amapatsira ndikuwononga ma erythrocyte amunthu, kutulutsa kuzizira, kutentha thupi, kuchepa magazi, ndi splenomegaly.P. falciparum imayambitsa matenda oopsa kwambiri kuposa mitundu ina ya plasmodial ndipo imachititsa imfa zambiri za malungo.P. falciparum ndi P. vivax ndi tizilombo toyambitsa matenda, komabe, pali kusiyana kwakukulu kwa malo pa kugawa mitundu.
Mwachikhalidwe, malungo amapezeka ndi kuwonetsera kwa zamoyo pa Giemsa zopakapaka zopakapaka zamagazi ozungulira, ndipo mitundu yosiyanasiyana ya plasmodium imasiyanitsidwa ndi mawonekedwe awo mu erythrocyte yomwe ili ndi kachilombo.Njirayi imatha kuzindikira zolondola komanso zodalirika, koma zikangochitidwa ndi akatswiri odziwa ma microscope pogwiritsa ntchito ma protocol omwe amafotokozedwa, omwe amapereka zopinga zazikulu kumadera akutali ndi osauka padziko lapansi.
Mayeso a Malaria Pf/Pv Ag Rapid apangidwa kuti athetse zopingazi.Amagwiritsa ntchito ma antibodies enieni a P. falciparum Histidine Rich Protein-II (pHRP-II) ndi P. vivax Lactate Dehydrogenase (Pv-LDH) kuti azindikire ndi kusiyanitsa matenda ndi P. falciparum ndi P. vivax.Mayeso amatha kuchitidwa ndi anthu osaphunzitsidwa kapena aluso pang'ono, opanda zida za labotale
MFUNDO
Mayeso a Malaria Pf/Pv Ag Rapid ndi kuyesa kwapambuyo kwa chromatographic immunoassay.Zigawo zoyeserera za mizereyo zimakhala ndi: 1) pad yamtundu wa burgundy yokhala ndi anti-Pv-LDH antibody yolumikizidwa ndi golide wa colloid (Pv-LDH-golide conjugates) ndi anti-pHRP-II antibody ya mbewa yolumikizidwa ndi golide wa colloid (pHRP-II-golide wokhala ndi ma conjugates awiri), 2 band cell balugate (Pv-LDH-gold conjugates) ndi (C gulu).Gulu la Pv limakutidwa kale ndi mbewa ina yolimbana ndi Pv-LDH yodziwira matenda a Pv, gulu la Pf limakutidwa ndi ma anti-pHRP-II a polyclonal anti-pHRP-II kuti azindikire matenda a Pf, ndipo gulu la C limakutidwa ndi mbuzi anti-mbewa IgG.
Pakuyesa, kuchuluka kokwanira kwachitsanzo cha magazi kumaperekedwa mu chitsime chachitsanzo (S) cha kaseti yoyesera, chotchinga cha lysis chimawonjezedwa ku buffer bwino (B).Chosungiracho chimakhala ndi zotsukira zomwe zimayendetsa maselo ofiira a magazi ndikutulutsa ma antigen osiyanasiyana, omwe amasuntha ndi capillary kupyola mzere womwe uli mu kaseti.Pv-LDH ngati zoperekedwa pachitsanzozi zimalumikizana ndi Pv-LDH-golide zolumikizira.Immunocomplex imatengedwa pa nembanemba ndi anti-Pv-LDH anti anti-PDH, kupanga gulu la burgundy mtundu wa Pv, kuwonetsa zotsatira za mayeso a Pv.Kapenanso, pHRP-II ngati ipezeka pachitsanzoyo imalumikizana ndi ma conjugates a golide a pHRP-II.Ma immunocomplex amatengedwa pa nembanemba ndi anti-pHRP-II anti-pHRP-II antibodies, kupanga gulu lamtundu wa burgundy Pf, kuwonetsa zotsatira zabwino za Pf.Kusakhalapo kwa magulu aliwonse oyeserera kukuwonetsa zotsatira zoyipa.Mayesowa ali ndi chowongolera chamkati (C band) chomwe chimayenera kuwonetsa gulu lamtundu wa burgundy wa immunocomplex wa mbuzi anti-mouse IgG / mbewa IgG (anti-Pv-LDH ndi anti-pHRPII) -ophatikiza golide mosasamala kanthu za kukula kwamitundu pamagulu aliwonse oyesa.Apo ayi, zotsatira zake ndizolakwika ndipo chitsanzocho chiyenera kuyesedwanso ndi chipangizo china.
