Mayeso a HIV / TP Antibody (Trilines)

Mayeso a HIV / TP Antibody (Trilines)

Mtundu: Mapepala Osadulidwa

Chizindikiro: Bio-mapper

Tsamba la deta: RC0211

Chitsanzo: WB/S/P

Kumverera: 99.70%

Zachidziwitso: 99.50%

Kuwunika magawo aukadaulo a kuzindikira treponema pallidum antibody (anti TP) ndi anti-AIDS virus (anti-HIV 1/2) ndi DIGFA.Njira zingapo zowongolera khalidwe labwino ndi 5863 seramu kapena zitsanzo za plasma za odwala zidadziwika ndi makhadi oyesa a DIGFA ndi enzyme immunoassay (EIA) kuchokera kwa opanga atatu motsatana.Kukhudzika, kutsimikizika, kuzindikira bwino komanso mawonekedwe amakadi oyesa a DIGFA adawunikidwa ndi ukadaulo wa EIA monga zofotokozera.Zotsatira Zapadera za makadi oyesa a TP ndi HIV 1/2 DIGFA mu sera zambiri zowongolera khalidwe zinali 100%;Kumverera kwa makadi oyesa a anti TP ndi anti HIVI1/2DIGFA anali 80.00% ndi 93.33% motsatana;Kuzindikira bwino kunali 88.44% ndi 96.97% motsatana.Zodziwika bwino za makadi oyesa a TP ndi anti HIV 1/2 DIGFA mu zitsanzo za 5863 serum (plasma) zinali 99.86% ndi 99.76% motsatira;Kukhudzidwa kunali 50.94% ndi 77.78%, motero;Kuzindikira bwino kunali 99.42% ndi 99.69% motsatana.Kutsiliza Khadi yoyesera ya DIGFA ili ndi chidwi chochepa komanso mtengo wokwera.Njira imeneyi ndi yoyenera kuwunika koyamba kwa odwala mwadzidzidzi, koma osati kuyesa kuyesa kwa opereka magazi.Ngati itagwiritsidwa ntchito powunika mwachangu opereka magazi mumsewu (galimoto yotolera magazi), iyenera kuphatikizidwa ndiukadaulo wa EIA.


Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Kufotokozera mwatsatanetsatane

Kuzindikira njira I chindoko
Kuzindikira kwa ma antibody a Treponema pallidum IgM
Kuzindikira kwa Treponema pallidum IgM antibody ndi njira yatsopano yodziwira chindoko m'zaka zaposachedwa.Ma antibody a IgM ndi mtundu wa immunoglobulin, womwe uli ndi ubwino wokhala ndi chidwi kwambiri, kuzindikira msanga, komanso kutsimikiza ngati mwana wosabadwayo ali ndi kachilombo ka Treponema pallidum.Kupanga ma antibodies enieni a IgM ndiye njira yoyamba yoyankhira chitetezo cha mthupi pambuyo pa matenda a chindoko ndi mabakiteriya ena kapena ma virus.Nthawi zambiri zimakhala zabwino kumayambiriro kwa matenda.Zimawonjezeka ndi chitukuko cha matendawa, ndiyeno antibody ya IgG imakwera pang'onopang'ono.
Pambuyo pa chithandizo chogwira ntchito, ma antibody a IgM adazimiririka ndipo ma IgG adapitilirabe.Pambuyo penicillin mankhwala, TP IgM mbisoweka mu gawo loyamba odwala chindoko ndi TP IgM zabwino.Pambuyo pa chithandizo cha penicillin, odwala TP IgM omwe ali ndi chindoko chachiwiri adazimiririka mkati mwa miyezi 2 mpaka 8.Kuphatikiza apo, kuzindikira kwa TP IgM ndikofunikira kwambiri pakuzindikirika kwa chindoko chobadwa nacho mwa ana akhanda.Chifukwa chakuti molekyulu ya IgM ndi yayikulu, antibody ya IgM ya amayi sangathe kudutsa mu placenta.Ngati TP IgM ili ndi HIV, mwanayo ali ndi kachilomboka.
Njira yodziwira chindoko II
Kuzindikira kwachilengedwe kwa mamolekyulu
M'zaka zaposachedwapa, biology ya maselo yakula mofulumira, ndipo teknoloji ya PCR yakhala ikugwiritsidwa ntchito kwambiri pazachipatala.Zomwe zimatchedwa PCR ndi polymerase chain reaction, ndiko kuti, kukulitsa mndandanda wosankhidwa wa spirochete DNA kuchokera ku zipangizo zosankhidwa, kuti awonjezere chiwerengero cha makope a spirochete a DNA osankhidwa, omwe angathandize kuti azindikire ndi ma probes enieni, ndikuwongolera mlingo wa matenda.
Komabe, njira yoyeserayi imafuna labotale yokhala ndi mikhalidwe yabwino kwambiri komanso akatswiri apamwamba, ndipo pali ma laboratories ochepa omwe ali ndi mulingo wapamwamba chotere ku China pakadali pano.Apo ayi, ngati pali kuipitsa, mudzaika Treponema pallidum, ndipo mutatha kukulitsa DNA, padzakhala Escherichia coli, zomwe zimakukhumudwitsani.Zipatala zina zazing'ono nthawi zambiri zimatsata mafashoni.Amapachika mtundu wa labotale ya PCR ndikudya ndi kumwa limodzi, zomwe zitha kukhala kudzinyenga okha.M'malo mwake, kuzindikira kwa chindoko sikufuna PCR, koma kuyezetsa magazi.

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