HCV(CMIA)

Matenda a chiwindi C sakudziwikabe.HCV ikabwerezanso m'maselo a chiwindi, imayambitsa kusintha kwa kapangidwe ndi ntchito ya maselo a chiwindi kapena kusokoneza kaphatikizidwe ka mapuloteni a maselo a chiwindi, zomwe zingayambitse kuwonongeka ndi necrosis ya maselo a chiwindi, zomwe zimasonyeza kuti HCV imawononga chiwindi mwachindunji ndipo imakhala ndi gawo la pathogenesis.Komabe, akatswiri ambiri a masamu amakhulupirira kuti cellular immunopathological reaction ingathandize kwambiri.Iwo adapeza kuti matenda a chiwindi C, monga hepatitis B, ali ndi ma CD3 + olowa m'maselo ake.Ma cell a Cytotoxic T (TC) amaukira makamaka ma cell omwe akukhudzidwa ndi matenda a HCV, omwe angayambitse kuwonongeka kwa maselo a chiwindi.

RIA kapena ELISA

Radioimmunodiagnosis (RIA) kapena enzyme-linked immunosorbent assay (ELISA) idagwiritsidwa ntchito pozindikira anti HCV mu seramu.Mu 1989, Kuo et al.adakhazikitsa njira ya radioimmunoassay (RIA) ya anti-C-100.Pambuyo pake, Ortho Company idapanga bwino kuyesa kwa enzyme-linked immunosorbent assay (ELISA) kuti izindikire anti-C-100.Onse njira ntchito recombinant yisiti anasonyeza HIV Antigen (C-100-3, puloteni otchulidwa NS4, munali 363 amino zidulo), pambuyo kuyeretsedwa, yokutidwa ndi pang'ono mabowo pulasitiki mbale, ndiyeno anawonjezera ndi anayesedwa seramu.Antigen ya virus imaphatikizidwa ndi anti-C-100 mu seramu yoyesedwa.Pomaliza, isotopu kapena enzyme yotchedwa mbewa anti human lgG monoclonal antibody imawonjezedwa, ndipo gawo lapansi limawonjezeredwa kuti mudziwe mtundu.