CHIDULE NDI KUTANTHAUZA MAYESERO
Mavairasi a Dengue, banja la ma serotypes anayi osiyana a ma virus (Den 1,2,3,4), ndi ma virus osakwatiwa, ophimbidwa, a RNA omwe ali ndi malingaliro abwino.Ma virus amafalitsidwa ndi udzudzu wa banja la Stegemyia, lomwe limaluma masana, makamaka Aedes aegypti, ndi Aedes albopictus.Masiku ano, anthu oposa 2.5 biliyoni okhala m’madera otentha a ku Asia, Africa, Australia, ndi America ali pa ngozi ya matenda a dengue.Pafupifupi anthu 100 miliyoni amadwala matenda a dengue fever komanso anthu 250,000 omwe amaika moyo pachiwopsezo cha matenda a dengue hemorrhagic fever amapezeka chaka chilichonse padziko lonse1-3.
Kuzindikira kwa serological kwa ma antibody a IgM ndi njira yodziwika kwambiri yodziwira matenda a dengue virus.Posachedwapa, kuzindikirika kwa ma antigen omwe amatulutsidwa panthawi yobwerezabwereza kachilombo kwa wodwala yemwe ali ndi kachilombo kunawonetsa zotsatira zabwino kwambiri.Zimathandizira kuzindikira kuyambira tsiku loyamba pambuyo pa kutentha kwa thupi mpaka tsiku la 9, pamene gawo lachipatala la matendawa latha, motero limalola chithandizo chofulumira kuikidwa mwamsanga4-.Mayeso a Dengue IgG / IgM Rapid amapangidwa kuti azindikire kufalikira kwa antigen ya dengue mu seramu, plasma kapena magazi athunthu.Kuyesako kutha kuchitidwa ndi anthu osaphunzitsidwa kapena aluso pang'ono, opanda zida za labotale.
MFUNDO
Dengue IgG/IgM Rapid Test ndi lateral flow chromatographic immunoassay.Kaseti yoyesera imakhala ndi: 1) chotupa chamtundu wa burgundy chokhala ndi ma antigen a dengue ophatikizana ndi golide wa colloid (ma conjugates a dengue) ndi ma conjugates a kalulu a IgG-golide, 2) mzere wa nitrocellulose wokhala ndi magulu awiri oyesera (G ndi M bandi) ndi control band).Gulu la G limakutidwa kale ndi anti-anti-dengue virus, M band imakutidwa ndi anti-dengue virus, ndipo C band idakutidwa kale ndi anti rabbit IgG.
Pamene chiwerengero chokwanira cha chitsanzo choyesera chaperekedwa mu chitsime cha kaseti yoyesera, chitsanzocho chimasuntha ndi capillary action kudutsa kaseti.Kachilombo ka anti-dengue ka IgG ngati kakupezeka pachitsanzochi kumalumikizana ndi ma conjugates a dengue.The immunocomplex ndiye anagwidwa ndi reagent yokutidwa pa G bandi, kupanga burgundy wachikuda G gulu, kusonyeza dengue HIV IgG HIV zotsatira ndi kutanthauza matenda posachedwapa kapena kubwereza.Kachilombo ka anti-dengue ka IgM, ngati kapezeka pachitsanzocho, amalumikizana ndi ma conjugates a dengue.The immunocomplex ndiye anagwidwa ndi reagent pre- TACHIMATA pa M band, kupanga burgundy wachikuda M band, kusonyeza dengue HIV IgM HIV zotsatira zabwino ndi kusonyeza matenda atsopano.
Kusakhalapo kwa magulu aliwonse oyesera (G ndi M) kumasonyeza zotsatira zoipa.Mayesowa ali ndi ulamuliro wamkati (C band) womwe uyenera kuwonetsa gulu lamtundu wa burgundy wa immunocomplex wa mbuzi anti rabbit IgG/rabbit IgG-gold conjugate mosasamala kanthu za kukula kwa mtundu pamagulu aliwonse a T.Apo ayi, zotsatira zake ndizolakwika ndipo chitsanzocho chiyenera kuyesedwanso ndi chipangizo china.
