CHIDULE NDI KUTANTHAUZA MAYESERO
Chikungunya ndi matenda osowa ma virus omwe amafalitsidwa mwa kulumidwa ndi udzudzu wa Aedes aegypti.Amadziwika ndi zidzolo, kutentha thupi, ndi kupweteka kwa mafupa (arthralgias) komwe nthawi zambiri kumatenga masiku atatu kapena asanu ndi awiri.Dzinali limachokera ku liwu la Chimakonde lotanthauza "chimene chimapindika" ponena za kaimidwe kowerama komwe kamapangidwa chifukwa cha zizindikiro za nyamakazi za matenda.Zimapezeka nthawi yamvula m'madera otentha padziko lapansi, makamaka ku Africa, South-East Asia, kum'mwera kwa India ndi Pakistan.Zizindikiro zake nthawi zambiri zimakhala zosadziwika bwino zomwe zimawonedwa ndi dengue fever.Zowonadi, matenda a dengue ndi chikungunya adanenedwa ku India.Mosiyana ndi dengue, kutulutsa magazi m'thupi sikochitika kawirikawiri ndipo nthawi zambiri matendawa ndi matenda odziletsa okha.Chifukwa chake ndikofunikira kwambiri kusiyanitsa dengue ndi matenda a CHIK.CHIK imapezeka potengera kusanthula kwa serological ndi kudzipatula kwa ma virus mu mbewa kapena chikhalidwe cha minofu.IgM immunoassay ndiye njira yothandiza kwambiri yoyesera labu.Chikungunya IgG/IgM Rapid Test imagwiritsa ntchito ma antigen omwe amachokera ku mapuloteni ake, amazindikira IgG/IgM anti-CHIK mu seramu ya odwala kapena plasma mkati mwa mphindi 20.Mayeso atha kuchitidwa ndi osaphunzitsidwa kapena
ogwira ntchito zocheperako, opanda zida zovuta za labotale.
MFUNDO
Chikungunya IgG/IgM Rapid Test is a lateral flow chromatographic immunoassay.Kaseti yoyesera imakhala ndi: 1) kaseti yamtundu wa burgundy yokhala ndi ma antigen a Chikungunya ophatikizana ndi golide wa colloid (ma conjugates a dengue) ndi ma conjugates a kalulu a IgG-golide, 2) mzere wa nitrocellulose wokhala ndi magulu awiri oyesera (G ndi M band (C band control).Gulu la G limakutidwa kale ndi anti-antibody kuti lizindikire kachilombo ka IgG anti-Chikungunya, M band imakutidwa ndi anti-Chikungunya virus, ndipo C band idakutidwa kale ndi mbuzi anti rabbit IgG.
Pamene chiwerengero chokwanira cha chitsanzo choyesera chaperekedwa mu chitsime cha kaseti yoyesera, chitsanzocho chimasuntha ndi capillary action kudutsa kaseti.IgG anti-Chikungunya virus ngati ikupezeka pachitsanzo ichi imalumikizana ndi ma conjugates a Chikungunya.Ma reagents a manambala osiyanasiyana a batch sangathe kugwiritsidwa ntchito mosinthana. The immunocomplex ndiye anagwidwa ndi reagent TACHIMATA pa G bandi, kupanga burgundy wachikuda G gulu, kusonyeza Chikungunya HIV IgG HIV zotsatira ndi kusonyeza posachedwapa kapena kubwereza matenda.Kachilombo ka IgM anti-Chikungunya, ngati kapezeka pachitsanzocho, amalumikizana ndi ma conjugates a Chikungunya.The immunocomplex imatengedwa ndi reagent yomwe idakutidwa kale pa M band, ndikupanga gulu la mtundu wa burgundy M, kuwonetsa zotsatira za mayeso a Chikungunya virus IgM ndikuwonetsa matenda atsopano.Kusakhalapo kwa magulu aliwonse oyesera (G ndi M) kumasonyeza zotsatira zoipa.Mayesowa ali ndi ulamuliro wamkati (C band) womwe uyenera kuwonetsa gulu lamtundu wa burgundy wa immunocomplex wa mbuzi anti rabbit IgG/rabbit IgG-gold conjugate mosasamala kanthu za kukula kwa mtundu pamagulu aliwonse a T.Apo ayi, zotsatira zake ndizolakwika ndipo chitsanzocho chiyenera kuyesedwanso ndi chipangizo china.
