Kufotokozera mwatsatanetsatane
Chindoko Tp ndi bakiteriya spirochete, amene ndi tizilombo toyambitsa matenda a chindoko venereal.Ngakhale kuti chiwerengero cha chindoko chikucheperachepera ku United States pambuyo pa kufalikira kwa chindoko, chiŵerengero cha chindoko ku Ulaya chakhala chikukwera kuchokera mu 1986 mpaka 1991.The World Health Organization lipoti 12 miliyoni atsopano milandu mu 1995. Pakali pano, mlingo wabwino wa chindoko serological kuyezetsa HIV anthu amene ali ndi kachilombo kamene kakukwera posachedwapa.
Kuzindikira kofulumira kwa ma syphilis antibody kuphatikiza ndi njira yoyesera ya mbali ya chromatography immunoassay.
Zida zoyesera zikuphatikiza: 1) cholumikizira chagolide cha Tp antigen IgG chomwe chimaphatikiza golide wofiirira (Tp conjugate) ndi akalulu.
2) Nitrocellulose membrane strip band yokhala ndi test band (T) ndi control band (C band).T band idakutidwa kale ndi antigen ya Tp yosagwirizana ndi conjugate, ndipo C band idakutidwa kale ndi anti rabbit IgG antibody.
Pamene voliyumu yokwanira ya zitsanzo imagawidwa mu dzenje lachitsanzo, chitsanzocho chimasuntha pa katoni ndi capillary action mu katoni.Ngati anti Tp antibody ilipo mu chitsanzo, imamanga ku Tp conjugate.Chitetezo cha mthupi ichi chimatengedwa pa nembanemba ndi antigen ya Tp yophimbidwa, ndikupanga gulu lofiirira la T, kuwonetsa zotsatira zabwino za antibody ya Tp.Kusowa kwa T band kumasonyeza kuti zotsatira zake ndi zoipa.Kuyesa kuphatikiza kuwongolera mkati (gulu C) kuyenera kuwonetsa gulu lofiirira lofiira la mbuzi anti rabbit IgG/rabbit IgG golide conjugate ya immune complex, mosasamala kanthu za T-band yake.Kupanda kutero, zotsatira zoyesa ndizosavomerezeka ndipo chipangizo china chiyenera kugwiritsidwa ntchito.
Zosinthidwa Mwamakonda Anu
Njira Yopangira Mapepala Osadulidwa Mwachangu
