Mayeso Ofulumira a TOXO IgG/IgM

Njira yoyendera
Pali njira zitatu zazikulu zodziwira toxoplasmosis: matenda a pathogenic, matenda ammunological ndi matenda a maselo.Kuwunika kwa tizilombo kumaphatikizapo kuzindikiritsa kwa histological, kulowetsedwa kwa nyama ndi kudzipatula, komanso chikhalidwe cha maselo.Njira zodziwika bwino za serological zikuphatikizapo kuyesa kwa utoto, kuyesa kwa hemagglutination kosalunjika, kuyesa kwa antibody kwa immunofluorescence ndi enzyme yolumikizidwa ndi immunosorbent assay.Kuzindikira kwa maselo kumaphatikizapo ukadaulo wa PCR ndi ukadaulo wa nucleic acid hybridization.
Kuyezetsa thupi kwa amayi oyembekezera kumaphatikizapo kuyezetsa kotchedwa TORCH.TORCH ndi kuphatikiza kwa chilembo choyamba cha dzina lachingerezi la tizilombo toyambitsa matenda.Chilembo T chikuimira Toxoplasma gondii.(Zilembo zina zimayimira chindoko, kachilombo ka rubella, cytomegalovirus ndi herpes simplex virus motsatana.)
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Kufufuza kwa tizilombo toyambitsa matenda
1. Kuwunika kwachindunji kakang'ono kakang'ono ka magazi a wodwalayo, m'mafupa kapena cerebrospinal fluid, pleural ndi ascites, sputum, bronchoalveolar lavage fluid, amadzimadzi amadzimadzi, amniotic fluid, ndi zina zotero. osati apamwamba.Angagwiritsidwenso ntchito mwachindunji immunofluorescence kudziwa Toxoplasma gondii mu zimakhala.
2. Katemera wa nyama kapena chikhalidwe cha minofu Tengani madzi a m'thupi kapena kuyimitsidwa kwa minofu kuti mukayesedwe ndikukathira m'mimba mwa mbewa.Matenda amatha kuchitika ndipo tizilombo toyambitsa matenda tingapezeke.Pamene m'badwo woyamba wa inoculation uli woipa, uyenera kuperekedwa mwakhungu katatu.Kapena chikhalidwe cha minofu (maselo a impso kapena nkhumba) kuti adzipatula ndikuzindikira Toxoplasma gondii.
3. Ukadaulo wosakanizidwa wa DNA Akatswiri apakhomo adagwiritsa ntchito ma 32P olembedwa ma probe okhala ndi ma DNA enieni a Toxoplasma gondii kwa nthawi yoyamba kuti achite kusakanizidwa kwa ma cell kapena minyewa ya DNA m'magazi am'magazi a odwala, ndipo adawonetsa kuti magulu kapena mawanga osakanikirana anali abwino.Zonse mwachindunji ndi kukhudzika zinali zapamwamba.Kuphatikiza apo, polymerase chain reaction (PCR) yakhazikitsidwanso ku China kuti izindikire matendawa, ndipo poyerekeza ndi kafukufuku wosakanizidwa, katemera wa nyama ndi njira zowunikira chitetezo chamthupi, zikuwonetsa kuti ndizodziwika kwambiri, zokhudzidwa komanso zachangu.
Kufufuza kwa Immunological
1. Ma antigen omwe amagwiritsidwa ntchito pozindikira antibody makamaka amaphatikizapo tachyzoite soluble antigen (cytoplasmic antigen) ndi membrane antigen.Ma antibody omwe adawonekera kale (omwe adadziwika ndi kuyezetsa madontho ndi mayeso osalunjika a immunofluorescence), pomwe omaliza adawonekera pambuyo pake (atazindikirika ndi mayeso osalunjika a hemagglutination, etc.).Nthawi yomweyo, njira zingapo zodziwira zimatha kuthandizana ndikuwongolera kuchuluka kwa kuzindikira.Chifukwa Toxoplasma gondii ikhoza kukhalapo m'maselo aumunthu kwa nthawi yaitali, n'zovuta kusiyanitsa matenda omwe alipo kapena matenda am'mbuyomu pozindikira ma antibodies.Ikhoza kuweruzidwa molingana ndi titer ya antibody ndi kusintha kwake kosinthika.
2. Kuzindikira antigen kumagwiritsidwa ntchito kuti azindikire tizilombo toyambitsa matenda (tachyzoites kapena cysts) m'maselo omwe akukhala nawo, metabolites kapena mankhwala a lysis (ma antigen ozungulira) mu seramu ndi madzi am'thupi mwa njira za immunological.Ndi njira yodalirika yodziwira matenda msanga komanso kuzindikira kotsimikizika.Akatswiri kunyumba ndi kunja akhazikitsa McAb ELISA ndi sangweji ELISA pakati pa McAb ndi multiantibody kuti azindikire kuyendayenda kwa antigen mu seramu ya odwala pachimake, ndi mphamvu ya 0.4 μ G/ml ya antigen.