Kufotokozera mwatsatanetsatane
Ganizirani zamtundu uliwonse wamunthu ngati zopatsirana ndikuzigwira pogwiritsa ntchito njira zodziwika bwino za biosafety.
Plasma
1.Sonkhanitsani chitsanzo cha magazi mu chubu lavenda, labuluu kapena lobiriwira pamwamba (lomwe lili ndi EDTA, citrate kapena heparin, motsatira Vacutainer®) ndi mitsempha.
2.Kusiyanitsa plasma ndi centrifugation.
3.Chotsani plasma mosamala mu chubu chatsopano cholembedwa kale.
Seramu
1.Sonkhanitsani chitsanzo cha magazi mu chubu chotolera pamwamba chofiira (chopanda anticoagulants mu Vacutainer®) mwa kubaya mitsempha.
2.Lolani magazi kuti atseke.
3.Kusiyanitsa seramu ndi centrifugation.
4.Chotsani mosamala seramu mu chubu chatsopano cholembedwa kale.
5.Yesani zitsanzo mwamsanga mutatha kusonkhanitsa.Sungani zitsanzo pa 2°C mpaka 8°C ngati sizinayesedwe msanga.
6.Sungani zitsanzo pa 2°C mpaka 8°C mpaka masiku asanu.Zitsanzozi ziyenera kusungidwa pa -20 ° C kuti zisungidwe nthawi yayitali
Magazi
Madontho a magazi athunthu amatha kupezeka poboola nsonga za chala kapena kubaya mtsempha.Osagwiritsa ntchito magazi aliwonse omwe ali ndi hemolized poyesa.Magazi athunthu amayenera kusungidwa mufiriji (2°C-8°C) ngati sanayesedwe msanga.Zitsanzozi ziyenera kuyesedwa mkati mwa maola 24 atatoledwa. Pewani maulendo angapo oundana.Musanayambe kuyezetsa, bweretsani zitsanzo zowundana ku kutentha pang'ono ndikusakaniza mofatsa.Zitsanzo zomwe zili ndi zinthu zowoneka bwino ziyenera kufotokozedwa ndi centrifugation musanayesedwe.
NJIRA YOYENERA
Khwerero 1: Bweretsani chitsanzo ndi zigawo zoyesera kuti zikhale kutentha ngati zili mufiriji kapena zachisanu.Mukasungunuka, sakanizani bwino musanayese.
Khwerero 2: Mukakonzeka kuyesa, tsegulani thumba pa notch ndikuchotsa chipangizocho.Ikani chipangizo choyesera pamalo oyera, ophwanyika.
Khwerero 3: Onetsetsani kuti mwalemba chipangizocho ndi nambala ya ID ya chitsanzo.
Khwerero 4: Pakuyezetsa magazi athunthu - Ikani dontho limodzi la magazi athunthu (pafupifupi 30-35 µL) pachitsime.- Kenako onjezani Madontho awiri (pafupifupi 60-70 µL) a Sample Diluent nthawi yomweyo.