Qrf Antibody Test Uncut Sheet

Mayeso a Qrf Antibody

Mtundu: Mapepala Osadulidwa

Chizindikiro: Bio-mapper

Zithunzi za REA0911

Chitsanzo: WB/S/P

Katemera woyeserera wa nkhosa pox virus amagwiritsidwa ntchito pozindikira ma antibodies a nkhosa mu seramu ya nkhosa, yomwe imatha kugwiritsidwa ntchito kuyesa chitetezo cha katemera wa pox kapena kuthandizira kuzindikira matendawa.Mfundo yake ndi yakuti zida zodziwira antibody za nkhosa za nkhosa zimapangidwa ndi kapepala ka nyukiliya ya antigen, zolembera za ma enzyme ndi zina zothandizira, ndipo mfundo ya enzyme-linked immunoassay (ELISA) imagwiritsidwa ntchito pozindikira kachilombo ka nkhosa mu seramu ya nkhosa.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Kufotokozera mwatsatanetsatane

Kuzindikira kwa antibody ya virus ya nkhosa kumapangidwa ndi microplate yomwe idakutidwa kale ndi antigen ya nyukiliya ya nkhosa, zolembera za ma enzyme ndi ma reagents ena othandizira, ndipo mfundo ya enzyme-linked immunoassay (ELISA) imagwiritsidwa ntchito kuzindikira kachilombo ka nkhosa mu seramu ya nkhosa.Panthawi yoyesera, seramu yolamulira ndi chitsanzo chomwe chiyenera kufufuzidwa chimawonjezedwa ku mbale ya microplate, ndipo ngati chitsanzocho chili ndi antibody pox pox pambuyo pa makulitsidwe, chidzamangidwa ku antigen pa mbale ya microplate, ndi zigawo zina zomwe sizimangika zidzachotsedwa pambuyo posamba;Kenako yonjezerani chikhomo cha enzyme kuti mumangirire ku antigen-antibody complex pa microplate plate;Zolemba za enzyme zosamangidwa zidachotsedwa ndikutsuka, ndipo gawo la gawo la TMB linawonjezeredwa ku zitsime, ndipo chopangidwa ndi buluu chinapangidwa ndi momwe ma conjugates a microplate amachitira, ndipo kuya kwa mtundu kunali kogwirizana bwino ndi kuchuluka kwa ma antibodies omwe ali mu zitsanzo.Pambuyo kuthetsa njira anawonjezera kuthetsa anachita, mankhwala anasanduka chikasu;Kuchuluka kwa kuyamwa pamachitidwe aliwonse kumatsimikiziridwa ndi owerenga ma microplate pamtunda wa 450 nm kuti adziwe ngati chitsanzocho chili ndi ma antibodies a pox pox.