CHIDULE NDI KUTANTHAUZA MAYESERO
Malungo ndi matenda oyambitsidwa ndi udzudzu, hemolytic, febrile omwe amakhudza anthu opitilira 200 miliyoni ndipo amapha anthu opitilira 1 miliyoni pachaka.Zimayambitsidwa ndi mitundu inayi ya Plasmodium: P. falciparum, P. vivax, P. ovale, ndi P. malariae.Ma plasmodia onsewa amapatsira ndikuwononga ma erythrocyte amunthu, kutulutsa kuzizira, kutentha thupi, kuchepa magazi, ndi splenomegaly.P. falciparum imayambitsa matenda oopsa kwambiri kuposa mitundu ina ya plasmodial ndipo imachititsa imfa zambiri za malungo.P. falciparum ndi P. vivax ndi tizilombo toyambitsa matenda, komabe, pali kusiyana kwakukulu kwa malo pa kugawa mitundu.
Mwachizoloŵezi, malungo amapezeka ndi kuwonetsera kwa zamoyo pa Giemsa zopakapaka zopakapaka zamagazi ozungulira, ndipo mitundu yosiyanasiyana ya plasmodium imasiyanitsidwa ndi maonekedwe awo mu erythrocytes1.Njirayi imatha kuzindikira zolondola komanso zodalirika, koma zikangochitidwa ndi akatswiri odziwa ma microscopist pogwiritsa ntchito ma protocol2, omwe amapereka zopinga zazikulu kumadera akutali komanso osauka padziko lapansi.
Malaria Pf / Pan Antigen Rapid Test Kit yapangidwa kuti ithetse zopingazi.Mayesowa amagwiritsa ntchito ma antibodies a monoclonal ndi polyclonal ku P. falciparum yeniyeni mapuloteni, Histidine Repeat Protein II (pHRP-II), ndi ma antibodies a monoclonal ku plasmodium Lactate Dehydrogenase (pLDH), puloteni yopangidwa ndi mitundu inayi ya plasmodium, motero imapangitsa kuti pakhale matenda amtundu umodzi wa plasmodium ndi plasmodium yosiyana. odya.Itha kuchitidwa ndi anthu osaphunzitsidwa kapena aluso pang'ono, popanda zida za labotale.
MFUNDO
Pf/Pan Malaria Rapid Test kit ndi lateral flow chromatographic immunoassay.Zigawo zoyeserera zimakhala ndi: 1) pad yamtundu wa burgundy yokhala ndi anti-PHRP-II antibody yolumikizidwa ndi golide wa colloid (pHRP II-golide conjugates) ndi anti-pLDH antibody yolumikizidwa ndi golide wa colloid (pLDH-golide conjugates),
2) chingwe cha nitrocellulose membrane chokhala ndi magulu awiri oyesera (Pan ndi Pv band) ndi gulu lowongolera (C band).Pan band imayikidwa kale ndi monoclonal anti-pLDH antibody yomwe imatha kuzindikirika ndi mitundu inayi ya plasmodia, gulu la Pf limakutidwa kale ndi ma antibodies a polyclonal anti-pHRP-II kuti azindikire matenda a Pf, ndipo gulu la C limakutidwa ndi mbuzi anti-mbewa IgG.
Pakuyesa, kuchuluka kokwanira kwachitsanzo cha magazi kumaperekedwa mu chitsime chachitsanzo (S) cha kaseti yoyesera, chotchinga cha lysis chimawonjezedwa ku buffer bwino (B).Chosungiracho chimakhala ndi zotsukira zomwe zimayendetsa maselo ofiira amagazi ndikutulutsa ma antigen osiyanasiyana a plasmodium, omwe amasuntha ndi capillary kupyola mzere womwe uli mu kaseti.PHRP-II ngati zoperekedwa pachitsanzozi zimalumikizana ndi ma conjugates a golide a pHRP II.The immunocomplex kenako amagwidwa pa nembanemba ndi anti-pHRPII anti-pHRPII antibodies, kupanga burgundy mtundu Pf gulu, kusonyeza Pf zabwino mayeso.pLDH ngati zoperekedwa pachitsanzozi zimagwirizana ndi ma conjugates agolide a pLDH.Immunocomplex imatengedwa pa nembanemba ndi anti anti pLDH antibody, ndikupanga gulu la Pan lamtundu wa burgundy, kuwonetsa zotsatira zabwino za plasmodium.Popanda gulu la Pan, zotsatira zabwino zoyeserera za plasmodia zina zitatuzi zitha kulimbikitsidwa.
Kusakhalapo kwa magulu aliwonse oyeserera (Pan ndi Pf) kukuwonetsa zotsatira zoyipa.Mayesowa ali ndi chowongolera chamkati (C band) chomwe chimayenera kuwonetsa gulu lamtundu wa burgundy la immunocomplex la anti-mbewa IgG / mbewa IgG (pHRP-II ndi pLDH-golide conjugates) mosasamala kanthu za kukula kwamitundu pamagawo aliwonse oyesa.Apo ayi, zotsatira zake ndizolakwika ndipo chitsanzocho chiyenera kuyesedwanso ndi chipangizo china.
