CHIDULE NDI KUTANTHAUZA MAYESERO
Malungo ndi matenda oyambitsidwa ndi udzudzu, hemolytic, febrile omwe amakhudza anthu opitilira 200 miliyoni ndipo amapha anthu opitilira 1 miliyoni pachaka.Zimayambitsidwa ndi mitundu inayi ya Plasmodium: P. falciparum, P. vivax, P. ovale, ndi P. malariae.Ma plasmodia onsewa amapatsira ndikuwononga ma erythrocyte amunthu, kutulutsa kuzizira, kutentha thupi, kuchepa magazi, ndi splenomegaly.P. falciparum imayambitsa matenda oopsa kwambiri kuposa mitundu ina ya plasmodial ndipo imachititsa imfa zambiri za malungo, ndipo ndi imodzi mwa tizilombo toyambitsa malungo tiwiri tofala kwambiri.
Mwachikhalidwe, malungo amapezeka ndi kuwonetsera kwa zamoyo pa Giemsa zopakapaka zopakapaka zamagazi ozungulira, ndipo mitundu yosiyanasiyana ya plasmodium imasiyanitsidwa ndi mawonekedwe awo mu erythrocyte yomwe ili ndi kachilombo.Njirayi imatha kuzindikira zolondola komanso zodalirika, koma zikangochitidwa ndi akatswiri odziwa ma microscope pogwiritsa ntchito ma protocol omwe amafotokozedwa, omwe amapereka zopinga zazikulu kumadera akutali ndi osauka padziko lapansi.
Pf Ag Rapid Test imapangidwa kuti ithetse zopinga izi.Imazindikira Pf yeniyeni antigen pHRP-II m'magazi amunthu.Itha kuchitidwa ndi anthu osaphunzitsidwa kapena aluso pang'ono, popanda zida za labotale.
MFUNDO
Pf Ag Rapid Test ndi lateral flow chromatographic immunoassay.Zigawo zoyeserera zimakhala ndi: 1) burgundy colored conjugate pad yokhala ndi anti-PHRP-II antibody yolumikizidwa ndi golide wa colloid (pHRP II-golide conjugates), 2) mzere wa nitrocellulose wokhala ndi bandi yoyesera (Pf) ndi gulu lowongolera (C band).Gulu la Pf limakutidwa kale ndi ma anti-pHRP-II a polyclonal anti-pHRP-II, ndipo gulu la C limakutidwa kale ndi anti-mbewa IgG.
Pakuyesa, kuchuluka kokwanira kwachitsanzo cha magazi kumaperekedwa mu chitsime chachitsanzo (S) cha kaseti yoyesera, chotchinga cha lysis chimawonjezedwa ku buffer bwino (B).Chosungiracho chimakhala ndi zotsukira zomwe zimatsuka maselo ofiira amagazi ndikutulutsa antigen ya pHRPII, yomwe imasuntha ndi capillary kupyola mzere womwe uli mu kaseti.PHRP-II ngati zoperekedwa pachitsanzozi zimalumikizana ndi ma conjugates a golide a pHRP II.Ma immunocomplex ndiye amagwidwa pa nembanemba ndi ma antibodies ophimbidwa kale a polyclonal antipHRP II, ndikupanga gulu la Pf lamtundu wa burgundy, zomwe zikuwonetsa zotsatira zabwino za Pf.
Kusakhalapo kwa gulu la Pf kukuwonetsa zotsatira zoyipa.Mayesowa ali ndi chowongolera chamkati (C band) chomwe chimayenera kuwonetsa gulu lamtundu wa burgundy la immunocomplex la anti-mbewa IgG / mbewa IgG (pHRP II-golide conjugates) mosasamala kanthu za kukula kwa mtundu pa gulu lililonse la Pf.Apo ayi, zotsatira zake ndizolakwika ndipo chitsanzocho chiyenera kuyesedwanso ndi chipangizo china.
