CHIDULE NDI KUTANTHAUZA MAYESERO
Matenda otchedwa lymphatic filariasis otchedwa Elephantiasis, omwe amayamba makamaka ndi W. bancrofti ndi B. malayi, amakhudza anthu pafupifupi 120 miliyoni m’mayiko 80.Matendawa amafalikira kwa anthu mwa kulumidwa ndi udzudzu womwe uli ndi kachilombo mkati mwake momwe tinthu tating'onoting'ono tomwe timayamwa kuchokera kwa munthu yemwe ali ndi kachilomboka timapanga mphutsi za gawo lachitatu.Nthawi zambiri, kukhudzana mobwerezabwereza ndi yaitali kukhudzana ndi kachilombo mphutsi chofunika kukhazikitsidwa kwa anthu matenda.
Kuzindikira kotsimikizika kwa parasitologic ndikuwonetsa ma microflariae mu zitsanzo zamagazi.Komabe, kuyesa kwa golide kumeneku kumakhala koletsedwa ndi kufunikira kwa kusonkhanitsa magazi usiku komanso kusowa kwa mphamvu zokwanira.Kuzindikira kwa ma antigen ozungulira kulipo pamalonda.Zothandiza zake ndizochepa kwa W. bancrofti.Kuphatikiza apo, microfilaremia ndi antigenemia zimayamba kuchokera miyezi kupita kuzaka pambuyo powonekera.
Kuzindikira ma antibodies kumapereka njira yodziwira matenda a filarial parasite.Kukhalapo kwa IgM ku ma antigen a parasite kumasonyeza kuti muli ndi kachilombo ka HIV, pamene IgG imafanana ndi matenda ochedwa kapena matenda apitalo.Kuphatikiza apo, kuzindikiritsa ma antigen osungidwa kumalola kuyesa kwa 'pan-filaria' kuti kugwire ntchito.Kugwiritsiridwa ntchito kwa mapuloteni ophatikizananso kumathetsa kusagwirizana ndi anthu omwe ali ndi matenda ena a parasitic.
Filariasis IgG/IgM Combo Rapid Test imagwiritsa ntchito zophatikiza zosungidwa
ma antigen kuti azindikire nthawi imodzi IgG ndi IgM ku W. bancrofti ndi B. malayi majeremusi popanda kuletsa kusonkhanitsa zitsanzo.
MFUNDO
Filariasis IgG/IgM Rapid Test Kit ndi lateral flow chromatographic immunoassay.Kaseti yoyesera ili ndi: 1) burgundy colored conjugate pad yomwe ili ndi recombinant W. bancrofti ndi B. malayi wamba antigens conjugated ndi golide colloid (Filariasis conjugates) ndi kalulu IgG-golide conjugates, 2) nitrocellulose ndi ntchentche ndi gulu loyang'anira G (M) Mzere ndi gulu loyang'anira G (M) ndi band yoyeseraM band imayikidwa kale ndi monoclonal anti-human IgM kuti izindikire IgM anti- W. bancrofti ndi B. malayi, G band imayikidwa kale ndi ma reagents kuti azindikire IgG anti-W.bancrofti ndi B. malayi, ndipo C band idakutidwa kale ndi anti rabbit IgG.
Pamene chiwerengero chokwanira cha chitsanzo choyesera chaperekedwa mu chitsime cha kaseti, chitsanzocho chimasuntha ndi capillary action kudutsa kaseti.W. bancrofti kapena B. malayi ma antibodies a IgM ngati alipo mu chitsanzo adzamanga ku Filariasis conjugates.The immunocomplex ndiye anagwidwa pa nembanemba ndi precoated anti-anthu IgM antibody, kupanga burgundy mtundu M band, kusonyeza W. bancrofti kapena B. malayi IgM zotsatira zabwino mayeso.
W. bancrofti kapena B. malayi ma antibodies a IgG ngati alipo mu chitsanzo adzagwirizana ndi Filariasis conjugates.The immunocomplex ndiye anagwidwa ndi chisanadze TACHIMATA reagents pa nembanemba, kupanga burgundy mtundu G gulu, kusonyeza W. bancrofti kapena B. malayi IgG zabwino mayeso zotsatira.
Kusakhalapo kwa magulu aliwonse oyeserera (M ndi G) kukuwonetsa zotsatira zoyipa.Mayesowa ali ndi chowongolera chamkati (C band) chomwe chimayenera kuwonetsa gulu lamtundu wa burgundy la immunocomplex of goat anti rabbit IgG/rabbit IgG-gold conjugate mosasamala kanthu za kukula kwa mtundu pamagulu aliwonse oyesa.Apo ayi, zotsatira zake ndizolakwika ndipo chitsanzocho chiyenera kuyesedwanso ndi chipangizo china.
