Kufotokozera mwatsatanetsatane
Canine parvovirus antigen quick test kit imagwiritsa ntchito mfundo ya sangweji ya antibody iwiri kuti izindikire bwino canine parvovirus antigen mu ndowe za agalu.Gulu loyamba la galu lotchedwa parvovirus antibody 1 linagwiritsidwa ntchito ngati chizindikiro, ndipo chigawo chodziwikiratu (T) ndi dera lolamulira (C) pa nembanemba ya nitrocellulose zidakutidwa ndi canine parvovirus antibody 2 ndi anti-nkhuku, motsatana.Pa nthawi yodziwika, chitsanzocho ndi chromatographic pansi pa zotsatira za capillary.Ngati chitsanzo choyesedwa chili ndi canine parvovirus antigen, antibody standard 1 imapanga antigen-antibody complex ndi canine parvovirus, ndipo imaphatikizana ndi canine parvovirus antibody 2 yokhazikika pamalo ozindikira panthawi ya chromatography kupanga "antibody 1-antigen-antibody 2" sangweji, zomwe zimapangitsa kuti pakhale gulu lofiira lofiira (T)Mosiyana ndi izi, palibe magulu ofiira ofiirira omwe amapezeka m'dera lodziwika (T);Mosasamala kanthu za kupezeka kapena kusakhalapo kwa canine parvovirus antigen pachitsanzocho, IgY complex ya nkhuku yokhazikika yagolide ipitilira kusanjidwa mmwamba kupita kudera lolamulira (C), ndipo gulu lofiira lofiirira lidzawonekera.Gulu lofiirira lofiirira lomwe limaperekedwa kumalo owongolera (C) ndiye muyeso wowunika ngati njira ya chromatography ndi yabwinobwino, komanso imagwiranso ntchito ngati mulingo wowongolera mkati mwa zotulutsa.