CHIDULE NDI KUTANTHAUZA MAYESERO
Kachilombo ka Zika (Zika): matenda opatsirana kwambiri akalumidwa ndi udzudzu wa Aedes, mayi ndi mwana, kuthiridwa magazi komanso kupatsirana pogonana.Ma antibody a IgG/IgM amapangidwa patatha sabata imodzi atayamba, motero kuzindikira kwa IgG/IgM ndikofunikira kwambiri koyambirira.
matenda a Zika virus.Zika amapezeka potengera kusanthula kwa serological komanso kudzipatula kwa ma virus mu mbewa kapena chikhalidwe cha minofu.IgM immunoassay ndiye njira yothandiza kwambiri yoyesera labu.Zika IgM/IgG Rapid Test imagwiritsa ntchito ma antigen omwe amachokera ku mapuloteni ake, imazindikira IgM/IgG anti-zika mu seramu ya odwala kapena plasma mkati mwa mphindi 15.Kuyesako kutha kuchitidwa ndi anthu osaphunzitsidwa kapena aluso pang'ono, popanda zida zovutirapo za labotale.
MFUNDO
Zika IgM/IgG Rapid Test ndi lateral flow chromatographic immunoassay.Kaseti yoyesera imakhala ndi: 1) pad yamtundu wa burgundy yokhala ndi antigen yolumikizananso ndi golide wa colloid (Zika conjugates) ndi ma conjugates a kalulu a IgG-golide, 2) chingwe cha nitrocellulose chokhala ndi magulu awiri oyesera (M ndi G magulu) ndi chowongolera. gulu (C gulu).M band idakutidwa kale ndi monoclonal anti-human IgM kuti izindikire IgM anti-Zika, G band idakutidwa kale ndi ma reagents kuti azindikire IgG anti-Zika, ndipo C band idakutidwa kale ndi anti-Zika. kalulu IgG.
Pamene chiwerengero chokwanira cha chitsanzo choyesera chaperekedwa mu chitsime cha kaseti yoyesera, chitsanzocho chimasuntha ndi capillary action kudutsa kaseti.Anti-Zika IgM ngati alipo mu chitsanzo adzamanga kwa Zika conjugates.The immunocomplex kenako amagwidwa pa nembanemba ndi anti-munthu IgM antibody, kupanga burgundy mtundu M band, kusonyeza Zika IgM zotsatira zabwino.
Anti-Zika IgG ngati ilipo mu fanizo idzagwirizana ndi ma conjugates a Zika.Immunocomplex imatengedwa ndi ma reagents omwe anali atakutidwa kale pa nembanemba, ndikupanga gulu lamtundu wa burgundy G, kuwonetsa zotsatira za mayeso a Zika IgG.Kusakhalapo kwa magulu aliwonse oyeserera (M ndi G) kukuwonetsa zotsatira zoyipa.Mayesowa ali ndi chowongolera chamkati (C band) chomwe chimayenera kuwonetsa gulu lamtundu wa burgundy la immunocomplex of goat anti rabbit IgG/rabbit IgG-gold conjugate mosasamala kanthu za kukula kwa mtundu pamagulu aliwonse oyesa.Apo ayi, zotsatira zake ndizolakwika ndipo chitsanzocho chiyenera kuyesedwanso ndi chipangizo china.
