Dengue fever ndi matenda opatsirana omwe amayamba chifukwa cha kachilombo ka dengue, komwe kumafalikira kwa anthu kudzera mu udzudzu.Zafala kwambiri padziko lonse lapansi, zomwe zimayambitsa matenda mamiliyoni ambiri komanso kufa kwa masauzande ambiri chaka chilichonse.Zizindikiro za malungo a dengue ndi kutentha thupi kwambiri, kupweteka kwa mutu, kupweteka m’malo olumikizirana mafupa ndi m’minyewa, ndipo zikavuta kwambiri, kungachititse kuti munthu azitaya magazi komanso kuwononga chiwalo chake.Chifukwa cha kufalikira kwake mwachangu komanso kofala, matenda a dengue akuwopseza kwambiri thanzi la anthu komanso moyo wapadziko lonse lapansi.
Kuti muzindikire msanga ndi kuletsa kufalikira kwa matenda a dengue, kuyezetsa ma virus mwachangu komanso molondola kwakhala kofunika kwambiri.Pachifukwa ichi, zida zowunikira mwachangu zimagwira ntchito yofunika kwambiri.Ndiwosavuta kugwiritsa ntchito, zida zoyezera mwachangu zomwe zimathandiza mabungwe azachipatala komanso ofufuza za miliri kuti adziwe mwachangu ngati anthu ali ndi kachilombo ka dengue.Pogwiritsira ntchito zida zodziwira matenda zimenezi, madokotala ndi ochita kafukufuku amatha kutulukira msanga anthu amene ali ndi kachilomboka ndi kuwapatula, n’kuyamba kulandira chithandizo choyenera komanso kupewa matenda a dengue fever.Chifukwa chake, zida zodziwira mwachangu ndizofunika kwambiri pakupewa komanso kuwongolera kufalikira kwa matenda a dengue fever.
Mfundo Yogwira Ntchito ndi Kagwiritsidwe Ntchito ka Rapid Diagnostic Kit
Mfundo Zofunikira za Antibody-Antigen Reaction
Ma antibody-antigen reaction ndi mfundo yofunika kwambiri mu immunology yomwe imagwiritsidwa ntchito pozindikira komanso kumanga ma antigen.Ma antibodies amamanga ma antigen kuti apange chitetezo chamthupi, njira yomangiriza yomwe imayendetsedwa ndi kukopana komanso kugwirizana pakati pa ma antibodies ndi ma antigen.Pankhani yoyesa mayeso a dengue fever, ma antibodies amamanga ma antigen kuchokera ku kachilombo ka dengue, zomwe zimapangitsa kuti pakhale zowoneka bwino za chitetezo chamthupi.
· Njira Yoyeserera ya Diagnostic Kit
Khwerero 1: Bweretsani chitsanzo ndi zigawo zoyesera kuti zikhale kutentha ngati zili mufiriji kapena zachisanu.Mukasungunuka, sakanizani bwino musanayese.
Khwerero 2: Mukakonzeka kuyesa, tsegulani thumba pa notch ndikuchotsa chipangizocho.Ikani chipangizo choyesera pamalo oyera, ophwanyika.
Khwerero 3: Onetsetsani kuti mwalemba chipangizocho ndi nambala ya ID ya chitsanzo.
Gawo 4: Kuyezetsa magazi athunthu
- Ikani dontho limodzi la magazi athunthu (pafupifupi 30-35 µL) pachitsimepo.
- Kenako onjezerani madontho awiri (pafupifupi 60-70 µL) a Sample Diluent nthawi yomweyo.
Kwa mayeso a seramu kapena plasma
- Dzazani chotsitsa cha pipette ndi chitsanzo.
- Kugwira chodontha choyimirira, perekani dontho limodzi (pafupifupi 30-35 µL) lachitsanzo pachitsimepo ndikuwonetsetsa kuti mulibe thovu la mpweya.
-Kenako onjezani madontho awiri (pafupifupi 60-70 µL) a Sample Diluent nthawi yomweyo.
Khwerero 6: Zotsatira zitha kuwerengedwa pakadutsa mphindi 20.Zotsatira zabwino zitha kuwoneka pakanthawi kochepa ngati mphindi imodzi.
Musawerenge zotsatira pambuyo pa mphindi 30. Kuti musasokonezeke, tayani chipangizo choyesera mutatanthauzira zotsatira.
· Kutanthauzira kwa Zotsatira za Assay
1. ZOTSATIRA ZOSAVUTA: Ngati gulu la C lokha lipangidwa, kuyesa kumasonyeza kuti mlingo wa dengue Ag mu chitsanzo sichidziwika.Zotsatira zake zimakhala zoipa kapena zosagwirizana.
2. ZOTSATIRA ZABWINO: Ngati magulu onse a C ndi T apangidwa, kuyezetsa kumasonyeza kuti chitsanzocho chili ndi dengue Ag.Zotsatira zake zimakhala zabwino kapena zowonongeka.Zitsanzo zokhala ndi zotsatira zabwino ziyenera kutsimikiziridwa ndi njira zina zoyesera monga PCR kapena ELISA ndi zofukufuku zachipatala zisanatsimikizidwe bwino.
3. YOSAVUTA: Ngati palibe gulu la C lomwe lapangidwa, kuyesako kumakhala kosavomerezeka mosasamala kanthu za kukula kwa mtundu pa T band monga zasonyezedwera pansipa.Bwerezani kuyesa ndi chipangizo chatsopano.
Ubwino wa BoatBio Dengue Rapid Diagnostic Kit
· Kuthamanga
1. Kuchepetsa Nthawi Yoyesera:
Chida chodziwira matenda chimagwiritsa ntchito ukadaulo woyeserera mwachangu, kulola kusanthula kwachitsanzo ndi kupanga zotsatira kumalizidwa mkati mwa mphindi 20.
Poyerekeza ndi njira zama labotale azikhalidwe, zidazo zimafupikitsa nthawi yoyesera, kukulitsa luso lantchito.
2. Kupeza zotsatira mu nthawi yeniyeni:
Chida chodziwira matenda chimapereka zotsatira zenizeni nthawi yomweyo mutangomaliza kukonza ndi kuchitapo kanthu.
Izi zimathandiza akatswiri azachipatala kuti apange matenda ndi zisankho mwachangu, kufulumizitsa kuwunika kwa matenda ndi njira zamankhwala.
· Kukhudzika ndi Kukhazikika
1. Kumverera Kwamphamvu:
Mapangidwe a zidazi amawathandiza kuzindikira kupezeka kwa kachilombo ka dengue mozindikira kwambiri.
Ngakhale mu zitsanzo zokhala ndi ma virus ochepa, zidazo zimazindikira kachilomboka, ndikupangitsa kulondola kwa matenda.
2. Zapamwamba:
Ma antibodies a zidawa amawonetsa kutsimikizika kwambiri, kuwalola kuti azilumikizana makamaka ndi kachilombo ka dengue.
Kusiyanitsa kumeneku kumathandizira zida kuti zizitha kusiyanitsa matenda a dengue virus ndi ma virus ena okhudzana nawo
(monga kachilombo ka Zika, kachilombo ka yellow fever), kuchepetsa kusadziwika bwino ndi zolakwika zabodza.
· Kusavuta Kugwiritsa Ntchito
1. Njira Zosavuta:
Zida zowunikira nthawi zambiri zimakhala ndi njira zowongoka, zomwe zimathandiza ogwiritsa ntchito kudziwa momwe angagwiritsire ntchito.
Masitepe omveka bwino komanso achidule akuphatikizidwa, kuphatikiza kuwonjezera kwachitsanzo, kusakanikirana kwa reagent, kuchitapo kanthu, ndi kutanthauzira kwa zotsatira.
2. Palibe Chofunikira Pazida Zovuta Kapena Labu:
Zida zowunikira nthawi zambiri sizifuna zida zovuta kapena labu kuti zigwiritsidwe ntchito ndikuwerenga zotsatira.
Kusunthika komanso kusinthasintha kumeneku kumapangitsa kuti zidazo zikhale zoyenera pazochitika zosiyanasiyana, kuphatikiza madera akutali kapena malo azachipatala omwe ali ndi zinthu zochepa.
Mwachidule, Dengue Rapid Diagnostic Kit imapereka zabwino monga kufulumira, kukhudzika, kukhazikika, komanso kugwiritsa ntchito mosavuta, ndikupangitsa kuti ikhale chida chofunikira kwambiri chodziwira kachilombo ka dengue moyenera komanso moyenera m'malo osiyanasiyana.
Malangizo a Zamankhwala
Nthawi yotumiza: Aug-16-2023