CHIDULE NDI KUTANTHAUZA MAYESERO
Visceral leishmaniasis, kapena Kala-azar, ndi matenda omwe amafalitsidwa chifukwa cha mitundu ingapo ya L. donovani.Bungwe la World Health Organisation (WHO) akuti matendawa akhudza anthu pafupifupi 12 miliyoni m'maiko 88.Amafalikira kwa anthu polumidwa ndi ntchentche za phlebotomus, zomwe zimatengera matenda chifukwa chodya nyama zomwe zili ndi kachilomboka.Ngakhale kuti ndi matenda amene amapezeka m’mayiko osauka, kum’mwera kwa Ulaya, ndiwo afala kwambiri mwa anthu odwala AIDS.Kuzindikiritsa zamoyo za L. donovani kuchokera m'magazi, m'mafupa, chiwindi, ma lymph nodes kapena ndulu kumapereka njira yotsimikizirika yodziwira matenda.Kuzindikira kwa serological kwa anti-L.donovani IgM imapezeka kuti ndi chizindikiro chabwino kwambiri cha acute Visceral leishmaniasis.Mayesero omwe amagwiritsidwa ntchito kuchipatala akuphatikizidwa ndi ELISA, antibody ya fulorosenti kapena mayeso a agglutination mwachindunji 4-5.Posachedwapa, kugwiritsidwa ntchito kwa L. donovani yeniyeni mapuloteni mu mayeso bwino tilinazo ndi mwachindunji kwambiri.
Mayeso a Leishmania IgG/IgM Combo Rapid ndi mayeso ophatikizananso ndi mapuloteni otengera serological, omwe amazindikira ma antibodies a IgG ndi IgM ku L. Donovani nthawi imodzi.Mayesowa amapereka zotsatira zodalirika mkati mwa mphindi 15 popanda zida zilizonse.
MFUNDO
Leishmania IgG/IgM Rapid Test ndi lateral flow chromatographic immunoassay.Kaseti yoyesera imakhala ndi: 1) pad yamtundu wa burgundy yokhala ndi recombinant L. donovani antigen yolumikizidwa ndi golide wa colloid (Leishmania conjugates) ndi ma conjugates a kalulu IgG-golide, 2) mzere wa nitrocellulose wokhala ndi magulu awiri oyesera (T1 ndi T2 magulu) ndi gulu lowongolera (C band).Gulu la T1 limakutidwa kale ndi monoclonal anti-human IgM kuti azindikire anti-L.donovani IgM, T2 gulu ndi precoated ndi reagents kuzindikira odana L.donovani IgG, ndipo C band idakutidwa kale ndi anti rabbit IgG.
Pamene chiwerengero chokwanira cha chitsanzo choyesera chaperekedwa mu chitsime cha kaseti, chitsanzocho chimasuntha ndi capillary action kudutsa kaseti.L. donovani IgM ngati ikupezeka pachitsanzoyo ilumikizana ndi ma conjugate a Leishmania.The immunocomplex ndiye anagwidwa pa nembanemba ndi pre- TACHIMATA anti-anthu IgM antibody, kupanga burgundy mtundu T1 gulu, kusonyeza L. donovani IgM zotsatira zabwino mayeso.L. donovani IgG ngati ikupezeka pachitsanzoyo idzalumikizana ndi ma conjugate a Leishmania.The immunocomplex ndiye anagwidwa ndi pre- TACHIMATA reagents pa nembanemba, kupanga burgundy mtundu T2 gulu, kusonyeza L. donovani IgG zotsatira zabwino mayeso.
Kusakhalapo kwa T band (T1 ndi T2) kukuwonetsa zotsatira zoyipa.Mayesowa ali ndi chowongolera chamkati (C band) chomwe chimayenera kuwonetsa gulu lamtundu wa burgundy la immunocomplex la anti rabbit IgG/rabbit IgG-gold conjugate mosasamala kanthu za kukula kwa mtundu pamagulu aliwonse a T.Apo ayi, zotsatira zake ndizolakwika ndipo chitsanzocho chiyenera kuyesedwanso ndi chipangizo china.
