CHIDULE NDI KUTANTHAUZA MAYESERO
Mavairasi a Dengue, banja la ma serotypes anayi osiyana a ma virus (Den 1,2,3,4), ndi ma virus osakwatiwa, ophimbidwa, a RNA omwe ali ndi malingaliro abwino.Ma virus amafalitsidwa ndi udzudzu wa banja la Stegemyia, lomwe limaluma masana, makamaka Aedes aegypti, ndi Aedes albopictus.Masiku ano, anthu oposa 2.5 biliyoni okhala m’madera otentha a ku Asia, Africa, Australia, ndi America ali pa ngozi ya matenda a dengue.Pafupifupi anthu 100 miliyoni amadwala matenda a dengue fever ndi matenda 250,000 a malungo oika moyo pangozi a dengue amatuluka chaka chilichonse padziko lonse lapansi.
Kuzindikira kwa serological kwa ma antibody a IgM ndi njira yodziwika kwambiri yodziwira matenda a dengue virus.Posachedwapa, kuzindikirika kwa ma antigen omwe amatulutsidwa panthawi yobwerezabwereza kachilombo kwa wodwala yemwe ali ndi kachilombo kunawonetsa zotsatira zabwino kwambiri.Zimathandizira kuzindikira kuyambira tsiku loyamba pambuyo pa kuyambika kwa malungo mpaka tsiku la 9, pamene gawo lachipatala la matendawa latha, motero limalola chithandizo chamankhwala mwamsanga mwamsanga4-.Mayeso a Dengue NS1 Rapid Test amapangidwa kuti azindikire kufalikira kwa dengue antigen mu seramu. , plasma kapena magazi athunthu.Kuyesako kutha kuchitidwa ndi anthu osaphunzitsidwa kapena aluso pang'ono, opanda zida za labotale.
MFUNDO
Mayeso a Dengue NS1 Rapid Test ndi lateral flow chromatographic immunoassay.Kaseti yoyesera ili ndi: 1) kaseti kofiira kofiira kokhala ndi mbewa yolimbana ndi dengue NS1 antigen yolumikizidwa ndi golidi wa colloid (Dengue Ab conjugates), 2) chingwe cha nitrocellulose chokhala ndi bandi yoyesera (T band) ndi gulu lowongolera (C gulu).T band idakutidwa kale ndi anti-dengue NS1 antigen, ndi C band
imakutidwa ndi anti-mbewa IgG antibody.Ma antibodies ku antigen ya dengue amazindikira ma antigen ochokera ku serotypes zonse zinayi za kachilombo ka dengue.
Pamene chiwerengero chokwanira cha chitsanzo choyesera chaperekedwa mu chitsime cha chitsanzo cha kaseti, chitsanzocho chimasuntha ndi capillary action kudutsa kaseti yoyesera.Dengue NS1 Ag ngati ilipo pachitsanzoyo ilumikizana ndi ma conjugates a Dengue Ab.The immunocomplex kenako amagwidwa pa nembanemba ndi mbewa antiNS1 antibody, kupanga burgundy mtundu T band, kusonyeza zotsatira zabwino Dengue Ag.
Kusakhalapo kwa T band kukuwonetsa zotsatira zoyipa.Mayesowa ali ndi chowongolera chamkati (C band) chomwe chiyenera kuwonetsa gulu lamtundu wa burgundy la immunocomplex la anti-mouse IgG/mouse IgG-gold conjugate mosasamala kanthu za kukhalapo kwa T band yachikuda.Apo ayi, zotsatira zake ndizolakwika ndipo chitsanzocho chiyenera kuyesedwanso ndi chipangizo china.
