CHIDULE NDI KUTANTHAUZA MAYESERO
Kolera ndi matenda opatsirana kwambiri omwe amadziwika ndi kutaya madzi ambiri am'thupi ndi ma electrolyte chifukwa cha kutsegula m'mimba kwambiri.The etiological agent wa kolera amadziwika kuti Vibrio cholerea (V. Cholerae), bakiteriya gram negative, amene nthawi zambiri amapatsira anthu kudzera madzi oipitsidwa ndi chakudya.
Mitundu ya V. Cholerae imagawidwa m'magulu angapo a serogroups pamaziko a O antigens.Magulu ang'onoang'ono O1 ndi O139 ndi ofunika kwambiri chifukwa onse angayambitse mliri ndi mliri wa kolera.Ndikofunikira kudziwa mwachangu kupezeka kwa V. cholerae O1 ndi O139 m'zitsanzo zachipatala, madzi, ndi chakudya kuti kuyang'anira koyenera komanso njira zodzitetezera zitha kuchitidwa ndi akuluakulu azaumoyo.
Mayeso a Kolera Ag Rapid atha kugwiritsidwa ntchito mwachindunji ndi anthu osaphunzitsidwa kapena aluso pang'ono ndipo zotsatira zake zimapezeka pasanathe mphindi 10, popanda zida zolemetsa za labotale.
MFUNDO
Cholera Ag Rapid Test ndi lateral flow chromatographic immunoassay.Kaseti yoyesera ili ndi: 1) pad yamtundu wa burgundy conjugate yokhala ndi monoclonal anti-V.Ma antibodies a Kolera O1 ndi O139 ophatikizidwa ndi golidi wa colloid (O1/O139-antibody conjugates) ndi ma conjugates a kalulu a IgG-golide, 2) mzere wa nitrocellulose nembanemba wokhala ndi magulu awiri oyesera (1 ndi 139 band) ndi gulu lowongolera (C band).Gulu la 1 limakutidwa kale ndi monoclonal anti-V.Cholera O1 antibody.Gulu la 139 ndi lopangidwa kale ndi monoclonal anti-V.Cholera O139 Antibody.C band idakutidwa kale ndi anti-mbewa IgG antibody.
Pamene chiwerengero chokwanira cha chitsanzo cha mayeso chiyikidwa mu chitsime cha kaseti choyesera, chitsanzocho chimasuntha ndi capillary action kudutsa kaseti.V. Cholera O1/O139 antigen ngati ilipo mu chitsanzo idzagwirizana ndi O1/O139-antibody conjugate yagolide.Immunocomplex iyi imatengedwa pa nembanemba ndi anti-V yomwe idakutidwa kale.Cholera O1/O139 antibody, kupanga gulu loyesera lamtundu wa burgundy, kuwonetsa zotsatira za mayeso a Cholera O1/O139.Kusakhalapo kwa gulu loyesera kukuwonetsa zotsatira zoyipa.
Mayesowa ali ndi chowongolera chamkati (C band) chomwe chikuyenera kuwonetsa gulu lamtundu wa burgundy la immunocomplex la anti-mbewa IgG/ mbewa IgG-golide conjugate mosasamala kanthu za kukula kwa mtundu pagulu loyesera.Apo ayi, zotsatira zake ndizolakwika ndipo chitsanzocho chiyenera kuyesedwanso ndi chipangizo china.
