CHIDULE NDI KUTANTHAUZA MAYESERO
Chikungunya ndi matenda osowa ma virus omwe amafalitsidwa mwa kulumidwa ndi udzudzu wa Aedes aegypti.Amadziwika ndi zidzolo, kutentha thupi, ndi kupweteka kwa mafupa (arthralgias) komwe nthawi zambiri kumatenga masiku atatu kapena asanu ndi awiri.Dzinali linachokera ku liwu la Chimakonde lotanthauza “chimene chimapindika” ponena za kaimidwe kowerama komwe kamapangika chifukwa cha zizindikiro za nyamakazi.Zimachitika nthawi yamvula
m'madera otentha padziko lapansi, makamaka ku Africa, South-East Asia, kum'mwera kwa India ndi Pakistan.Zizindikiro zake nthawi zambiri zimakhala zosadziwika bwino zomwe zimawonedwa ndi dengue fever.Zowonadi, matenda a dengue ndi chikungunya adanenedwa ku India.Mosiyana ndi dengue, kutulutsa magazi m'thupi sikochitika kawirikawiri ndipo nthawi zambiri matendawa ndi matenda odziletsa okha.Choncho ndikofunikira kwambiri
kusiyanitsa matenda a dengue ndi matenda a CHIK.CHIK imapezeka potengera kusanthula kwa serological ndi kudzipatula kwa ma virus mu mbewa kapena chikhalidwe cha minofu.IgM immunoassay ndiye njira yothandiza kwambiri yoyesera labu.Chikungunya IgG/IgM Rapid Test imagwiritsa ntchito ma antigen omwe amachokera ku mapuloteni ake, amazindikira IgG/IgM anti-CHIK mu seramu ya odwala kapena plasma mkati mwa mphindi 20.Mayeso atha kuchitidwa ndi osaphunzitsidwa kapena
ogwira ntchito zocheperako, opanda zida zovuta za labotale.
MFUNDO
Chikungunya IgG/IgM+NS1 antigen Rapid Test is a lateral flow chromatographic immunoassay.Kaseti yoyeserera imakhala ndi Mzere wa IgG/IgM ndi mzere wa NS1.
Mzere wa IgG/IgM: 1) cholembera chamtundu wa burgundy chokhala ndi ma antigen a Chikungunya ophatikizana ndi golide wa colloid (ma conjugates a dengue) ndi ma conjugates a kalulu a IgG-golide,2) mzere wa nitrocellulose wokhala ndi magulu awiri oyesera (G ndi M band) ndi gulu lowongolera (C band).G band imayikidwa kale anti-Chikungunya virus, M band imakutidwa ndi anti-Chikungunya virus, ndipo C band idakutidwa kale ndi mbuzi anti-Rabbit IgG.
NS1 strip: 1) burgundy colored conjugate pad yokhala ndi anti-Chikungunya NS1 antigen yolumikizidwa ndi golidi wa colloid (Chikungunya Ab conjugates), 2) mzere wa nitrocellulose wokhala ndi bandi yoyeserera (T band) ndi gulu lowongolera (C band).T band idakutidwa kale ndi anti-Chikungunya NS1 antigen, ndipo C band idakutidwa ndi anti-mbewa IgG antibody.
Mzere wa IgG/IgM: Pamene chiŵerengero chokwanira cha chitsanzo cha mayeso chaperekedwa mu chitsime cha kaseti yoyesera, chitsanzocho chimasuntha ndi capillary action kudutsa kaseti.IgG anti-Chikungunya virus ngati ikupezeka pachitsanzo ichi imalumikizana ndi ma conjugates a Chikungunya.The immunocomplex imatengedwa ndi reagent yokutidwa pa G band, kupanga burgundy mtundu G band, kusonyeza Chikungunya virus IgG HIV zotsatira ndi kutanthauza matenda posachedwapa kapena kubwereza.Kachilombo ka IgM anti-Chikungunya, ngati kakupezeka pachitsanzochi, amalumikizana ndi ma conjugates a Chikungunya.The immunocomplex imatengedwa ndi reagent yomwe idakutidwa kale pa M band, ndikupanga gulu la mtundu wa burgundy M, kuwonetsa zotsatira za mayeso a Chikungunya virus IgM ndikuwonetsa matenda atsopano.Kusowa kwa magulu aliwonse oyeserera (G ndi M) kukuwonetsa zotsatira zoyipa. Mayesowa ali ndi chowongolera chamkati (C band) chomwe chikuyenera kuwonetsa gulu lamtundu wa burgundy la immunocomplex la goat anti rabbit IgG/
kalulu IgG-golide conjugate mosasamala kanthu za kukula kwa mtundu pamagulu aliwonse a T.Apo ayi, zotsatira zake ndizolakwika ndipo chitsanzocho chiyenera kuyesedwanso ndi chipangizo china.
Mzere wa NS1: Pamene chiwerengero chokwanira cha chitsanzo cha mayeso chaperekedwa mu chitsime cha kaseti, chitsanzocho chimasuntha ndi capillary action kudutsa kaseti yoyesera.Chikungunya NS1 Ag ngati ikupezeka pachitsanzochi ilumikizana ndi ma conjugates a Chikungunya Ab.The immunocomplex kenako anagwidwa pa nembanemba ndi precoated kalulu anti-NS1 antibody, kupanga burgundy mtundu T band, kusonyeza Chikungunya Ag zotsatira zabwino mayeso.Kusakhalapo kwa T band kukuwonetsa zotsatira zoyipa.Mayesowa ali ndi chowongolera chamkati (C band) chomwe chiyenera kuwonetsa gulu lamtundu wa burgundy la immunocomplex la anti-mouse IgG/mouse IgG-gold conjugate mosasamala kanthu za kukhalapo kwa T band yachikuda.Apo ayi, zotsatira zake ndizolakwika ndipo chitsanzocho chiyenera kuyesedwanso ndi chipangizo china.
